PacBio (NASDAQ: PACB), a leading developer of high-quality, highly accurate sequencing solutions, today announced that it has begun taking orders for its groundbreaking Kinnex RNA kits, which measure full-length RNA, single-cell RNA, and 16S Increases throughput for rRNA sequencing on PacBio’s long-read sequencing system to enable large-scale studies at resolutions difficult to achieve with short-read RNA sequencing.
“The new family of Kinnex kits for RNA sequencing is designed to provide customers with a cost-effective, end-to-end kitted solution to support their research,” said Christian Henry, Pacbio’s President and CEO. “Revio System HiFi brings scale to whole genome sequencing. Our customers have said the same for RNA sequencing, and these Kinnex kits deliver millions of full-length reads per run. This is a game changer for the RNA sequencing market.”
Kinnex kits are based on the MAS-Seq method, which ligates short DNA fragments into long, HiFi-ready libraries. Short-read sequencing has difficulty covering the entire transcript, while long-read sequencing of a transcript traditionally results in untapped sequencing capacity. With Kinnex, customers can connect transcripts to long libraries for HiFi sequencing, increasing throughput and making long-read RNA sequencing more cost-effective.
The new Kinnex Single-Cell RNA Kit builds on the existing Single Cell 3′ Kit for MAS-Seq, adds the 10x Genomics 5′ Kit and additional support for library multiplexing.
The Kinnex Full-Length RNA Kit offers full-length RNA sequencing with abundant data and flexible sample multiplexing. The kit can yield 40 million reads
15 million reads per Revio system per 25M SMRT cells and Sequel II and IIe systems per 8M SMRT cells. Additionally, the end-to-end workflow includes access to PacBio’s SMRT Link software for full-length RNA and isoform data analysis.
“Kinnex kits now allow us to generate HiFi long-read transcriptome data at much higher throughput than our predecessor Iso-Seq, and to do so at the single-cell level. This is going to be very useful for annotating genic regions more precisely than short reads in our genome assembly and measuring their expression levels at single-cell resolution,” said Erich Jarvis, Chair of the Vertebrate Genome Project and Director of the Vertebrate Genome Lab.
Finally, the Kinnex 16S rRNA kit enables extraordinary resolution, high-quality microbiome sequencing at any scale. The kit will provide 60 million reads per 25M SMRT cell on Revio systems and 25 million reads per 8M SMRT cell on Sequel II and IIe systems. With a recommendation of 384-plexes per library, this new kit will allow full-length 16S rRNA sequencing at a competitive cost per sample compared to current standard short read sequencing.
“We are excited about the opportunity to drive real advances in microbiome studies and translational research for our customers with the Kinnex 16S rRNA kit,” said Dr. Shuiquan Tang, Director of Microbiome at Zymo Research. “The ability to perform full-length 16S sequencing on the PacBio system allows us to provide customers with higher resolution, species-level taxonomic data at a cost that is competitive for short-read sequencing.”
With the Kinnex kit offerings, Revio will provide customers with the ability to sequence up to 5,200 RNA samples per year for less than $500 USD per sample – a 16-fold increase in sample throughput over what was previously available to users of the PacBio RNA Seque II and IIe systems. . Three Kinnex kits sell for $5,700 USD at 12 reactions each
PacBio is now accepting orders for each kit and will begin shipping in December 2023.